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Rodney Rothstein
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Professor of Genetics and Development
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| Address: |
701 West 168th Street Room 1608 New York NY 10032 |
| Phone: |
212-305-1733 |
| Fax: |
212-923-2090 |
E-mail:
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rothstein@cancercenter.columbia.edu
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Education and Training:
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Ph.D. 1975, University of Chicago
Postdoctoral Fellow 1975-1977, University of Rochester
Postdoctoral Fellow 1977-1979, Cornell University
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Affiliations:
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 Department of Genetics and Development
Institute of Cancer Genetics
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Training Activities:
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Department of Genetics and Development
Integrated Program in Cellular, Molecular and Biophysical Studies
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Research Summary:
(800 words, max)
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Yeast genetics and cell biology cellular responses to DNA damage; recombination; control of genome stability. |
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Current Research:
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By using budding yeast as an experimental organism, we are able to study essential biological processes such as the mechanisms underlying the recognition and repair of DNA damage. The role that genetic recombination plays during repair is an integral part of our research.
We are exploring the biological response to DNA damage by studying a central recombination protein, Rad52, a ribonuclease reductase inhibitor that responds degrades after DNA damage, Sml1, and a topoisomerase helicase complex necessary for resistance to DNA damaging agents, Top3/Sgs1.
The availability of a complete gene disruption library is another tool that aids our research. By developing methods to enhance the utility of this resource, we are facilitating genome-wide analysis of not only budding yeast, but other species as well. |
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Publications:
(6 max)
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1. Feng, Q., Düring L., Antúnez de Mayolo, A., Lettier G., Lisby M., Erdeniz N., Mortensen H.U., Rothstein, R.: (2007) Rad52 and Rad59 exhibit both overlapping and distinct functions. DNA Repair 6(1): 27-37
2. Lettier G., Feng, Q., Antúnez de Mayolo, A., Erdeniz, N., Reid, R.J.D., Lisby, M., Mortensen, H.M. and Rothstein, R.: (2006) The role of DNA double-strand breaks in spontaneous homologous recombination in S. cerevisiae. PLoS Genet 2(11): e194
3. Alvaro D., Sunjevaric I., Reid R., Lisby M., and Rothstein R.: (2006) Systematic Hybrid LOH: a new method to reduce false positives and negatives during screening of yeast gene deletion libraries. Yeast 23: 1097-1106
4. Wagner, M., Price, G. and Rothstein, R.: (2006) The absence of Top3 reveals an interaction between the Sgs1 and Pif1 DNA helicases in Saccharomyces cerevisiae. Genetics 174: 555-573
5. Antúnez de Mayolo, A., Lisby, M., Erdeniz, N., Thybo, T., Mortensen, U.H., and Rothstein, R.: (2006) Multiple start codons and phosphorylation result in discrete Rad52 protein species . Nucleic Acids Res. 34: 2587-97
6. Thorpe, P.H., Marrero, V.A., Savitsky, M.H., Sunjevaric, I., Freeman, T.C., Rothstein, R.: ( 2006) Cells expressing murine RAD52 splice variants favor sister chromatid repair. Molec. Cell. Biol 26: 3752-63
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URL for lab page:
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http://www.rothsteinlab.com |
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