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Max  Gottesman
Max Gottesman
Revson Professor of Biochemistry & Molecular Biophysics & Microbiology


Address: 701 West 168th Street HHSC Room 914 New York NY 10032
Phone: 212-305-6900
Fax: 212-1741
E-mail:

meg8@columbia.edu

Education and Training:
M.D. 1960, Yale University
Ph.D. 1965, Yale University
Postdoctoral Fellow 1960-1964, National Institute of Health
Affiliations:
bullet  Department of Biochemistry & Molecular Biophysics
bullet  Department of Genetics & Development
bullet  Department of Microbiology
bullet  Institute of Cancer Research
Training Activities:
bullet  Department of Biochemistry & Molecular Biophysics
bullet  Department of Genetics and Development
Research Summary:
(800 words, max)
Regulation of transcription termination in E. coli and bacteriophage; role of cAMP in cell cycle progession and signal transduction in eukaryotes; vitamin A metabolism in the mouse.
Current Research:
The Gottesman Laboratory investigates the mechanism of transcription termination in E. coli and how termination affects other cellular processes. Blocking the release of a stalled transcription elongation complex leads to clashes with the replisome and the formation of lethal DNA double-strand breaks. Coupling of transcription to translation suppresses stalling. NusG links RNA polymerase and the first translating ribosome. The linkage is broken by tmRNA, which releases ribosomes at rare codons. The interactions among ribosomes, RNA polymerase and DNA polymerase are being investigated using genetic and biochemical approaches. In addition, the laboratory is probing the structure of the ribosome-NusG-RNA polymerase complex (in collaboration with Dr J. Frank).
In collaboration with Dr J. Gautier, we study the repair of DNA double-strand breaks in Xenopus extracts and in mammalian cells. We focus on the repair of interstrand DNA crosslinks, such as are generated by treatment with the antitumor agent, cisplatin.
The subcellular location of PKA is of critical importance in transmission of cAMP signals. How PKA anchoring proteins (AKAPs) affect cAMP signal transduction is being investigated in cultured cell lines and in mouse mode systems.
Publications:
(6 max)
1. Kim, H.C. and Gottesman, M.E.: (2004) Transcription Termination by Phage HK022 Nun Is Facilitated by C-terminal Lysine Residues.   J. Biol. Chem  279: 13412-7

2. Feliciello, A., Gottesman, M.E., and Avvedimento, E.V. : (2005) cAMP-PKA signaling to the mitochondria: protein scaffolds, mRNA and phosphatases.  Cellular Signalling   17: 279-287

3. Kim, H.C., Washburn, R.S, and Gottesman M.E. : (2006) Role of E. coli NusA in Phage HK022 Nun-mediated Transcription Termination.  J. Mol. Biol   359: 10-12

4. Björn M. Burmann B, Schweimer K, Luo X, Wahl MC, Stitt BL Gottesman ME, and Rösch P: (2010) A NusG:NusE complex links transcription and translation.  Science  (in press)

URL for lab page:
 

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